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OP Labs formerly Oxford Peptides.Batch HPLC tested at 99% purity.Store frozen long term or in fridge when ready to be used.Sold for research purposes only.Contact us for Wholesale Orders.Download COA here: 5Amino-1mq-COAPlease note if you have a different Batch ID please contact us for the latest COA.5-Amino-1MQSynonyms / Designations: 5-Amino-1-methylquinolinium, 5-Amino-1-MQ, NNMT inhibitor analogCAS Number: 42464-96-0Molecular Formula: C10H11N2 (monocationic quaternary ammonium)Molecular Weight: ~159.21 g/molPurity: ≥ 98 % (HPLC, typical)Appearance: Off-white to pale yellow crystalline powderPack Size: 10 mg (total)Storage: Desiccated, protected from light, stored at –20 °CSolubility: Soluble in water, dilute acids; soluble in polar aprotic solvents (e.g. DMSO)Description & Mechanism5-Amino-1MQ is a synthetic methylquinolinium derivative designed as a potent inhibitor of nicotinamide N-methyltransferase (NNMT). In biochemical research systems, it modulates metabolic signaling by interfering with NNMT catalytic activity, thereby influencing levels of methylated nicotinamide derivatives and related metabolites. Peptide Sciences 1NNMT is a cytosolic enzyme that catalyzes methyl transfer from S-adenosyl methionine (SAM) to nicotinamide and related substrates. Because of its metabolic role, NNMT is expressed in liver, adipose tissue, kidney, and other tissues. Peptide SciencesIn preclinical biochemical settings, inhibition of NNMT by compounds like 5-Amino-1MQ leads to alterations in intracellular NAD⁺ salvage pathways, shifts in methyl donor balance, and downstream metabolic signaling changes. Peptide SciencesApplications in ResearchAs a molecular probe to study NNMT function, regulation, and downstream metabolic pathwaysTo investigate metabolic flux, methylation dynamics, NAD⁺ salvage, and related bioenergetic phenomenaIn cellular assays, to test effects of NNMT inhibition on gene expression, metabolite profiling, or metabolic phenotypesIn animal / ex vivo experiments for mechanistic elucidation of metabolic regulation (e.g. adipose tissue, liver)Handling, Reconstitution & StabilityWeigh under inert/dry conditions; avoid moisture uptakeFor stock solutions, dissolve in water or dilute acid (e.g. 0.1 N HCl) or compatible solvent (e.g. DMSO) depending on assay needsFilter sterilize if needed (e.g. 0.22 µm) immediately prior to useAliquot and store stock solutions at –20 °C (or lower) to avoid repeated freeze–thawMonitor for precipitation or pH shifts before use in sensitive assaysSpecifications SummaryParameterTypical Value / RangePurity (HPLC)≥ 98%AppearanceOff-white to pale yellow powderMolecular Weight~159.21 g/molCharge StateMonocationic (quaternary ammonium)SolubilityWater, dilute acids, DMSOStorage–20 °C, desiccated, darkPack Size10 mgPrecautions & NotesBecause 5-Amino-1MQ is a small molecule inhibitor with ionic character, buffer composition, ionic strength, and pH in assays may influence its behavior (ion pairing, binding).It is advisable to run appropriate controls (vehicle, dose–response) to exclude non-specific effects in in vitro systems.For in vivo or ex vivo studies (if employed in nonclinical contexts), consider potential biodistribution, clearance, and off-target interactions in experimental design.Use of light, high temperature, or prolonged aqueous exposure may degrade or oxidize the compound; prepare fresh or store stocks under protective conditions.ReferencesNeelakantan H. et al. Selective and membrane-permeable small molecule inhibitors of nicotinamide N-methyltransferase reverse high-fat diet-induced obesity in mice. Biochemical Pharmacology, 2018.https://pubmed.ncbi.nlm.nih.gov/29225089/Kraus D. et al. Nicotinamide N-methyltransferase knockdown protects against diet-induced obesity. Nature, 2014.https://pubmed.ncbi.nlm.nih.gov/24739937/Pissios P. Nicotinamide N-methyltransferase: more than a vitamin B3 clearance enzyme. Trends in Endocrinology & Metabolism, 2017.https://pubmed.ncbi.nlm.nih.gov/28433686/Hong S. et al. Nicotinamide N-methyltransferase regulates hepatic nutrient metabolism through Sirt1 protein stabilization. Nature Medicine, 2015.https://pubmed.ncbi.nlm.nih.gov/25961501/Keywords: 5amino1mq, 5-amino-1mq, 5 amino.