OP Labs formerly Oxford PeptidesBatchHPLC tested at 99% purityStore frozen long term or in fridge when ready to be usedSold for research purposes onlyContact us for Wholesale OrdersDownload COA here: ACE-031_COA.pdfPlease note: if you have a different Batch ID, please contact us for the latest COA.ACE-031Synonyms / Designations: ACE-031, ActRIIB-Fc, Activin Type IIB receptor fusion protein, Myostatin-binding fusion proteinCAS Number: 1621169-52-5Molecular Formula: C133H227N43O33Molar Weight: 2956.5 g/molProtein Composition: Extracellular domain of human activin receptor type IIB (ActRIIB) fused to the Fc region of human IgG1Protein Classification: Recombinant fusion protein; soluble decoy receptorPurity: ≥ 99 % (HPLC / SEC, typical)Appearance: White to off-white lyophilised powderPack Size: 1 mg (total)Storage: Desiccated, protected from light, stored at –20 °CSolubility: Soluble in sterile water and buffered aqueous solutionsDescription & MechanismACE-031 is a recombinant fusion protein consisting of the extracellular ligand-binding domain of activin receptor type IIB (ActRIIB) linked to the Fc portion of human IgG1. It was developed as a structurally defined soluble receptor construct for experimental investigation of ligand–receptor interactions within the transforming growth factor-β (TGF-β) superfamily.In biochemical and cellular research systems, ACE-031 functions as a decoy receptor capable of binding select TGF-β family ligands, including myostatin (GDF-8) and activins, thereby preventing their interaction with endogenous cell-surface receptors. Experimental studies typically examine ligand binding affinity, downstream signalling modulation, and pathway inhibition under controlled conditions. Observed molecular responses are dependent on experimental design, ligand availability, and model system, and are used to explore signalling mechanisms rather than defined biological or physiological outcomes.Applications in Research• As a molecular tool for studying myostatin and activin ligand binding• Investigation of TGF-β superfamily signalling regulation• In vitro studies examining receptor–ligand sequestration mechanisms• Use as a reference construct in fusion protein and decoy receptor researchHandling, Reconstitution & Stability• Handle using appropriate protein-handling procedures• Reconstitute in sterile water or suitable buffered aqueous solution (e.g. PBS)• Gently swirl to dissolve; do not vortex• Filter sterilize if required (e.g. 0.22 µm) immediately prior to use• Aliquot and store reconstituted solutions at –20 °C (or lower)• Avoid repeated freeze–thaw cyclesSpecifications SummaryPurity: ≥ 99 %Appearance: White to off-white lyophilised powderProtein Type: Recombinant ActRIIB-Fc fusion proteinMolar Weight: 2956.5 g/molSolubility: Water, buffered aqueous solutionsStorage: –20 °C, desiccated, protected from lightPack Size: 1 mgPrecautions & Notes• Experimental behaviour of ACE-031 is influenced by ligand concentration, receptor expression, and model system• Glycosylation state and buffer composition may affect stability and binding characteristics• Fusion proteins may exhibit batch-dependent biophysical properties• Appropriate controls are recommended to distinguish specific ligand sequestration from non-specific effects• Intended strictly for laboratory research use; not for human or veterinary applicationReferencesLee S.J. et al. Regulation of muscle growth by multiple ligands signaling through activin type II receptors. Proceedings of the National Academy of Sciences, 2005.https://pubmed.ncbi.nlm.nih.gov/16330774/Attie K.M. et al. A single ascending-dose study of muscle regulator ACE-031 in healthy volunteers. Muscle & Nerve, 2013.https://pubmed.ncbi.nlm.nih.gov/23169607/Sako D. et al. Characterization of the ligand binding functionality of the extracellular domain of activin receptor type IIB. Journal of Biological Chemistry, 2010.https://pubmed.ncbi.nlm.nih.gov/20385559/Keywords: ACE-031, Ramatercept, ActRIIB-Fc, Myostatin Decoy Receptor, TGF-β Signalling, Recombinant Fusion Protein.
